Developing Chamber – Flat Bottom Chamber

 

This is the classical developing tank for thin-layer chromatography. It permits the plate to be developed under conditions of partial or complete saturation of the tank atmosphere with solvent vapours. The degree of layer preconditioning cannot be controlled unless additional accessories are used.

Developing Chamber – Twin Trough Chamber

 

The CAMAG Twin Trough Chamber offer several ways to specifically influence chromatogram development in order to improve it.

 

Low solvent consumption

20 mL of solvent are sufficient for a 20x20 cm chamber, 10 mL for the 20x10 cm chamber and 5 mL for a 10x10 cm chamber. This reduces not only solvent consumption but also disposal problems.

 

Reproducible preconditioning of the layer with solvent vapour

Developing solvent is placed in the trough opposite to the plate. Preconditioning can be performed with any solvent and for any duration. Development is started when developing solvent is placed into the trough with the plate.

Automatic Developing Chamber (ADC 2)

 

The Automatic Developing Chamber ADC 2 offers convenience, safety and reproducibility for isocratic developments of TLC/ HPTLC plates and foils with the format 20 x 10 cm.

 

How ADC 2 works

The Automatic Developing Chamber ADC 2 is the heart of an HPTLC system. It performs the development step fully automatically, reproducibly, and independent of environmental effects. The activity and pre-conditioning of the layer, chamber saturation, developing distance and final drying can be pre-set and automatically monitored by the ADC 2. Two modes of operation are possible: stand-alone with input of parameters via keypad, or remote operation from winCATS with process monitoring, documentation of operating parameters, and reporting.The ADC 2 with winCATS is compliant with the requirements of GMP/GLP and can be IQ/OQ qualified. If you want to use the instrument in a 21 CFR Part 11 environment, the option 21 CFR Part 11 «compliance ready» is required for each winCATS workstation.

 

Key features

  • Fully automatic development of 20 x 10 cm TLC/HPTLC plates.

  • A conventional 20 x 10 cm Twin Trough Chamber is used for development. This way chamber geometry and chromatographic conditions of already existing analytical procedures can be retained, but environmental and operational effects are excluded.

  • Operation in stand-alone mode or under winCATS.

  • The user is freed of all process monitoring responsibilities, operation is fully traceable.

  • The option «Humidity Control» allows reproducible chromatography at defined activity of the layer.

Horizontal Developing Chamber

 

In the CAMAG Horizontal Developing Chamber, the HPTLC plate is developed from both opposing sides towards the middle. This permits the number of samples to be doubled as compared with development in a tank, provided the separation distance of 45 mm, i.e. 50 mm minus 5 mm distance from the edge, is sufficient.

 

 

AMD 2 System – Automated Multiple Development

 

The CAMAG AMD procedure allows Thin-Layer chromatography to be utilized for tasks that could not be performed by TLC in the past.

 

How AMD 2 works

Only the AMD procedure can be successfully employed for reproducible gradient elution with silica gel as the stationary phase. In column liquid chromatography, gradient elution is common on reversed phases only because a silica gel column would call for a time consuming reconditioning or be irreversibly degraded, which is not acceptable in a technique depending on multiple use of the stationary phase. In Thin-Layer chromatography this is not relevant.

AMD = Automated Multiple Development

 

The AMD 2 with winCATS is compliant with the requirements of GMP/GLP and can be IQ/OQ qualified. If you want to use the instrument in a 21 CFR Part 11 environment, the option 21 CFR Part 11«compliance ready» is required for each winCATS workstation.

 

The principle of the CAMAG AMD procedure

  • The HPTLC plate is developed repeatedly in the same direction.· Each successive run extends over a longer solvent migration distance than the one before.

  • Between runs, the solvent is completely removed from the developing chamber and the layer is dried under vacuum.

  • Each successive run uses a solvent of lower elution strength than that of the one used before. In this way, a stepwise elution gradient is formed.

  • The combination of focusing effect and gradient elution results in extremely narrow bands. Their typical peak width is about 1 mm. This means that, with the available separation distance of 80 mm, up to 40 components can be completely resolved, i.e. with base line separation.

 

AMD 2 under winCATS

The AMD 2, like other computer controlled CAMAG instruments, communicates with winCATS. The gradient, made from up to 5 solvent bottles, is defined by input into a table in winCATS. Gradient and developing distance for each run can be shown graphically for verification. Then all individual runs of the developing program are performed fully automatic and monitored by winCATS.

 

Key features

  • Multiple developments, gradient elution.

  • Separation power improved over regular HPTLC by a factor 3.

  • Data input and monitoring through winCATS.

  • Utilizing time also after working hours.

smartAlert Solvent Front Monitor

 

smartALERT for dependable monitoring of TLC/HPTLC plate development in a glass development chamber.How smartALERT works· The desired developing distance is set by the user.· A light beam and a sensor monitor the selected distance.· As soon as the front has reached this position, visual and acoustic signals alert the operator. The «classical» way of developing a chromatogram in TLC and HPTLC is to place the plate in a chamber containing developing solvent. Driven by capillary action the developing solvent moves up the layer until the desired developing distance is reached. With the aid of a stop watch or a timer the chromatographer estimates when the development process is about to finish.

 

But this may happen: Focusing on other work while the TLC development is in progress, the lab technician forgets to look at the watch. As a result chromatography is stopped too late; the solvent moved up too far, the analysis is ruined and needs to be repeated.With smartALERT the chromatographer does not 'forget' the TLC plate in the developing chamber anymore. Most convenient!

 

Key features

  • Guarantees that the developing distance will always be accurate and according to the method.

  • Gives acoustic and visual notice when the mobile phase has reached the desired developing distance.

  • Replaces the timer or the stop watch.

  • Lets you concentrate on other lab work.

  • Is an intelligent and dependable assistant; yet very easy to operate with just one button.

  •  Works with tanks and for glass plates; sizes 20 x 20 cm, 20 x 10 cm, 10 x 10 cm.

  • Saves costs because the development is always 'done right'; no waste because of 'over-developed' plates.·

  • Allows several developments with several smart Alerts at the same time; four different alarm sounds can be selected.

  • Runs on batteries: up to 1000 developments.

HPTLC Vario System

 

Development with six different solvents can be tested side by side

 

Key features

 

  • Development with six different solvents can be tested side by side.

  • Sandwich as well as tank configuration can be simulated side by side, making results directly comparable.

  • Six different conditions of pre-equilibration, including relative humidity, can be tested simultaneously.

  • These variations of developing conditions can be freely combined.

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