Chromatogram development is the key step of the TLC/HPTLC process. Automatic development chambers allow to make the development step more reproducible and less dependent on human interaction. Chamber configuration, activation, developing distance, and final drying can be preset. The progress of the development step is monitored.
The primary focus of an automatic chamber ( CAMAG® HPTLC PRO Module DEVELOPMENT, CAMAG® Automatic Developing Chamber 2 (ADC 2) or CAMAG® AMD 2 System Automated Multiple Development ) is to make the development more reproducible and independent of human interaction. The layer is automatically brought in contact with the mobile phase and the migration is monitored. After completion of chromatography, the plate is dried. With an automatic chamber, the activity of the stationary phase can be adjusted. In the case of the CAMAG® HPTLC PRO Module DEVELOPMENT, the gas phase (from developing solvent or alternative solvent) can even be actively circulated during development to affect the separation of analytes.
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