It is an inherent advantage of TLC/HPTLC that all analytes remain stored on the plate and can be readily derivatized after chromatography. Analytes that do not respond to visible or UV light can be rendered detectable. In many cases, analytes or classes of analytes can be identified by specific reagents, enabling their selective detection.
In most cases, the derivatization reaction needs to be completed by heat treatment. Heating the plate at the desired temperature with a plate heater ( CAMAG® TLC Plate Heater 3) specifically designed for this purpose is highly recommended. A plate heating unit is an integral part of the CAMAG® HPTLC PRO Module DERIVATIZATION